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| Registros recuperados : 8 | |
| 1. |  | BAENA, M. M.; COSTA, A. C.; VIEIRA, G. R.; ROCHA, R. de F. B.; RIBEIRO, A. R. B.; IBELLI, A. M. G.; MEIRELLES, S. L. C. Heat tolerance responses in a Bos Taurus cattle herd raised in a Brazilian climate. Journal of Thermal Biology, v. 81, p. 162-169, 2019.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 2. | | ROCHA, R. de F. B.; OTTO, P. I.; GARCIA, A. O.; SANTOS, M. G. dos; SILVA, M. V. G. B.; MARTINS, M. F.; MACHADO, M. A.; PANETTO, J. C. do C.; CALUS, M. P. L.; GUIMARÃES, S. E. F. A genética envolvida na produção de oócitos e embriões em Gir leiteiro. Balde Branco, ano 59, n. 703, p. 56-59, 2024.| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | ROCHA, R. de F. B.; OTTO, P. I.; SILVA, M. V. G. B.; MARTINS, M. F.; MACHADO, M. A.; VERONEZE, R.; LEANDRO, F. D.; PEREIRA, S. M.; GUIMARÃES, S. E. F.; PANETTO, J. C. do C. Repeatability and random regression models to estimate genetic parameters for oocyte and embryo production in the Gir breed. Animal Production Science, v. 62, n. 17, p. 1661-1670, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | ROCHA, R. de F. B.; GARCIA, A. O.; OTTO, P. I.; SILVA, M. V. G. B.; MARTINS, M. F.; MACHADO, M. A.; PANETTO, J. C. do C.; GUIMARÃES, S. E. F. Runs of homozygosity and signatures of selection for number of oocytes and embryos in the Gir Indicine cattle. Mammalian Genome, v. 34, p. 482-496, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | ROCHA, R. de F. B.; GARCIA, A. O.; OTTO, P. I.; SANTOS, M. G. dos; SILVA, M. V. G. B.; MARTINS, M. F.; MACHADO, M. A.; PANETTO, J. C. do C.; GUIMARÃES, S. E. F. Single-step genome-wide association studies and post-GWAS analyses for the number of oocytes and embryos in Gir cattle. Mammalian Genome, v. 34, p. 497-508, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | OTTO, P. I.; GOBO, D. O. R.; OLIVEIRA, D.; BÖCK, M. J.; GARCIA, A. O.; SANTOS, M. G. dos; OTTO, T. M.; ROCHA, R. de F. B.; SILVA, M. V. G. B.; DALTRO, D. dos S.; NEGRI, R.; KLUSKA, S.; COSTA, K. A. Aplicações da genômica na produção animal. In: SIMPÓSIO DE INVESTIGAÇÃO EM PRODUÇÃO ANIMAL, 1., 2023, Santa Maria, RS. [Trabalhos apresentados]. Canoas, RS: Mérida Publishers, 2023. p. 95-112.| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | GARCIA, A. O.; OTTO, P. I.; GLATZL JUNIOR, L. A.; ROCHA, R. de F. B.; SANTOS, M. G. dos; OLIVEIRA, D. A. de; SILVA, M. V. G. B.; PANETTO, J. C. do C.; MACHADO, M. A.; VERNEQUE, R. da S.; GUIMARÃES, S. E. F. Pedigree reconstruction and population structure using SNP markers in Gir cattle. Journal of Applied Genetics, v. 64, p. 329-340, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | ROCHA, R. de F. B.; GARCIA, A. O.; SANTOS, M. G. dos; OTTO, P. I.; SILVA, M. V. G. B.; MARTINS, M. F.; MACHADO, M. A.; PANETTO, J. C. do C.; CALUS, M. P. L.; VANDENPLAS, J.; GUIMARÃES, S. E. F. Parent-of-origin effects for the number of oocytes and embryos in Gir cattle. Livestock Science, v. 280, 105412, 2024.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 8 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
03/07/2017 |
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Data da última atualização: |
03/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
FANÉ, R.; KASSOGUÉ, A.; TRAORÉ, D.; DAO, S.; MAÏGA, K.; BAH, A.; VALICENTE, F. H.; BARBANA, A. H. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Molecular characterization and insecticidal activities of Malian native crystalliferous Bacillus thuringiensis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
African Journal of Biotechnology, v. 16, n. 17, p. 983-988, 2017. |
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DOI: |
10.5897/AJB2017.15908 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to select the most active Bacillus thuringiensis (Bt) isolated from agricultural soils of Mali through the molecular characterization and the determination of insecticidal activities of the protein crystals, produced by these native isolates. Crystal proteins were extracted from B. thuringiensis culture, and characterized using the SDS-PAGE techniques. Their insecticidal activities were tested using third-instar larvae of Helicoverpa armigera in bioassay tests. The results showed that, of 62 B. thuringiensis treated, 52 isolates showed fragments varying between 10 and 140 kDa on 12% polyacrylamide gel. Cry1 and Cry2 protein crystals were recognized to be effective against Lepidoptera?s larvae, which were found in 21% of the tested isolates. In addition to these two expected crystal protein weights, other molecular weights were observed at different proportions, suggesting the presence of other cry genes in the local B. thuringiensis isolates. Four native B. thuringiensis isolates were able to kill 95 to 100% of H. armigera 3rd-instar larvae. Only one native of B. thuringiensis isolate was able to kill 100% of the H. armigera larvae. This is the first study for molecular characterization of Malian native B. thuringiensis isolates, showing the efficacy of the native B. thuringiensis against an important agricultural insect pest. |
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Thesagro: |
Bacteria; Controle biológico. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161312/1/Molecular-characterization-1.pdf
|
|
Marc: |
LEADER 02099naa a2200241 a 4500
001 2071896
005 2017-07-03
008 2017 bl uuuu u00u1 u #d
024 7 $a10.5897/AJB2017.15908$2DOI
100 1 $aFANÉ, R.
245 $aMolecular characterization and insecticidal activities of Malian native crystalliferous Bacillus thuringiensis.$h[electronic resource]
260 $c2017
520 $aThe objective of this work was to select the most active Bacillus thuringiensis (Bt) isolated from agricultural soils of Mali through the molecular characterization and the determination of insecticidal activities of the protein crystals, produced by these native isolates. Crystal proteins were extracted from B. thuringiensis culture, and characterized using the SDS-PAGE techniques. Their insecticidal activities were tested using third-instar larvae of Helicoverpa armigera in bioassay tests. The results showed that, of 62 B. thuringiensis treated, 52 isolates showed fragments varying between 10 and 140 kDa on 12% polyacrylamide gel. Cry1 and Cry2 protein crystals were recognized to be effective against Lepidoptera?s larvae, which were found in 21% of the tested isolates. In addition to these two expected crystal protein weights, other molecular weights were observed at different proportions, suggesting the presence of other cry genes in the local B. thuringiensis isolates. Four native B. thuringiensis isolates were able to kill 95 to 100% of H. armigera 3rd-instar larvae. Only one native of B. thuringiensis isolate was able to kill 100% of the H. armigera larvae. This is the first study for molecular characterization of Malian native B. thuringiensis isolates, showing the efficacy of the native B. thuringiensis against an important agricultural insect pest.
650 $aBacteria
650 $aControle biológico
700 1 $aKASSOGUÉ, A.
700 1 $aTRAORÉ, D.
700 1 $aDAO, S.
700 1 $aMAÏGA, K.
700 1 $aBAH, A.
700 1 $aVALICENTE, F. H.
700 1 $aBARBANA, A. H.
773 $tAfrican Journal of Biotechnology$gv. 16, n. 17, p. 983-988, 2017.
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Embrapa Milho e Sorgo (CNPMS) |
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